Bibcode : Natur. Four families of zebrafish were used for aCGH and each family consisted of the parents, two male offspring and two female offspring individuals. One of the main pigment cell types in zebrafish are the xanthaphores, which form a yellow strip of pigment that lies between the characteristic black pigment stripes formed by melanophores which give zebrafish their name.
Cytogenet Genome Res. Rev Fish Sci. Over the past decades, zebrafish Danio rerio has become an important laboratory model organism for many areas of research for examples see e. In summary, we found a good match overall between the number of males and females, as determined by visual and histological gonadal analysis, and the results from the microarray-based transcriptomic analysis.
Earlier, others polygenic sex determination system in zebrafish in Chelmsford indicated the possibility of a polygenic sex determination system for zebrafish based on a single set of experiment each see e. This small-bodied freshwater species is most commonly found in slow or stagnant waters, such as rivers, ponds and paddy fields [ 67 ].
In the female-biased group, the difference in mean male percentages for the first and second batches was 1. Unbiased offspring. The development of transgenic zebrafish lines that showed differential reporter signals between the developing female strong fluorescence and male weak fluorescence gonads [ — ] has opened up new possibilities to analyze the underlying processes.
Polymerase chain reaction PCR -based methods such as random amplified polymorphic DNA RAPD [ 86 ] and amplified fragment length polymorphism AFLP [ 87 ] are the most popular molecular methods for isolation of sex markers, but other procedures have also been used [ 88 ].
However, when selection pressure was removed at F 2 generation by performing a random mass cross, CV for F 3 generation family sex ratio returned to a level similar to that of unselected F 0 generation. Figure S3: Sex-linked FluoMEP markers obtained polygenic sex determination system in zebrafish in Chelmsford bulk segregant analysis performed on pooled male and female samples of guppy Poecilia reticulata.
Supporting Information Figure S1 Multifactorial selective breeding was carried out over a few generations for five lines of zebrafish to select for pairs that produced a sex-biased family. Environ Biol Fish.
A and rosy barb Puntius conchonius; B. Green lines depict genetic interactions between factors and pink lines depict protein—protein interactions. Stephanie A. Bulk segregant analysis The classical method to determine if a species is using  was performed using the male and female pooled gDNA chromosomal sex determination system is to analyze the sex ratio samples to screen for potentially sex-linked markers.
Other unusual systems include those of the swordtail fish [ clarification needed ] ;  the Chironomus midges [ clarification needed ] [ citation needed ] ; the juvenile hermaphroditism of zebrafish , with an unknown trigger;  and the platyfish , which has W, X, and Y chromosomes.
Kirankumar S, Pandian T Production and progeny testing of